Yes, the Garden of Eden still exists today. The Garden is the place that God made for Adam to live in, and from where they were driven after The Fall. The entrance to it is guarded so that it cannot be found, but it is located in the Middle East near the four rivers of the area of Eden: Pishon, Gihon, Euphrates, Tigris. The location of the first two is not known.
"So He drove the man out; and at the east of the Garden of Eden He stationed the cherubim and the flaming sword which turned every direction to guard the way to the tree of life," (Gen. 3:24).
The entrance to the garden is it guarded by cherubim so that people who might happen upon it would not be able to enter and thereby partake of the tree of life and live forever in their fallen state.